Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Philipp Lorenz
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:27
dcmA-gene with N-terminal pdu-microcompartment tag, codon optimised for E.coli (K12)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1446001_sequence (Version 1) |
Description
dcmA is a gene coding for the enzyme dichloromethane dehalogenase from Methylobacterium extorquens (DM4). This enzyme is a member of the glutathione-dependent-S-transferase family and catalyses the following reaction:DCM (dichloromethane) + 2H2O -> formaldehyde + 2 HCl
We have attached the N-terminal targeting sequence for the pdu-microcomartment to this part in order to allow for the insertion of this enzyme into the microcompartment.
Notes
The wild-type dcmA-gene has two EcoRI-restriction sites. We have therefore changed the restriction site sequences from GAATTC to GAATTT in order to make this part BioBrick compatible.Source
The sequence comes from the original gene in the genomic DNA of DM4. We have taken the seuqence from uniprot.| Sequence Annotation | Location | Component / Role(s) |
| N-terminal pdu-microcompartment tag dcmA from M. extorquens, codon optimised for E.coli K12 | 4,51 52,830 | tag feature/tag feature/cds CDS |