BBa_K1446002

BBa_K1446002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1446002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Philipp Lorenz
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:27

superfolder GFP with N-terminal pdu-microcompartment tag



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K1446002_sequence (Version 1)

    Description

    We have used superfolded GFP with the N-terminal pdu-microcompartment tag in order to get fluorescence images that would show localised spots of higher GFP-density, indicating that the microcompartment tag successfully inserts the attached protein into the microcompartments

    Notes

    We have designed Primers to amplify sfGFP from a plasmid, provided by Dr. Ciaran Kelly, in order to attach the microcompartment tag upstream of the sfGFP-coding region.

    Source

    We received a sample of sfGFP in a holding vector, provided by Dr. Ciaran Kelly.

    Sequence Annotation Location Component / Role(s)
    ribosome binding site
    pdu-microcompartment tag
    superfolder GFP coding sequence
    		14,19
    31,80
    81,831
    		feature/rbs ribosome_entry_site
    feature/tag tag
    feature/cds CDS

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1446002/1
     

    Attachments

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