BBa_K1452002

BBa_K1452002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1452002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Keoni Gandall

Date created: 2014-11-02 12:00:00

Date modified: 2015-05-08 01:10:29

Pos/neg selection (Bacillus)

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_K1452002_sequence (Version 1)

Description

This is a positive/negative selection cassette for Bacillus. If flanked by Bacillus integration sites and integration sites for any chosen DNA, that DNA can be integrated into Bacillus and then the selection cassette can be integrated over by whatever DNA you chose.

Notes

***the mazF toxin is unstable in plasmids without the mazE antitoxin***

Source

http://www.bgsc.org/viewdetail.php?bgscid=ECE194&Search=pDGieF
http://www.ncbi.nlm.nih.gov/pubmed/16714443

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lacI	31,1113	CDS, feature/protein
mazF	1363,1698	feature/protein, CDS
Pspac	1715,2003	feature/promoter, promoter
SpcR	2113,2865	CDS, feature/protein
lac operator	1722,1738	feature/operator, operator

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1452002/1

Member of these Collections

• iGEM Parts Registry