BBa_K1459002

BBa_K1459002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-09-30 11:00:00

Date modified: 2015-05-08 01:10:32

C-term of PmrB from Salmonella enterica

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1459002_sequence (Version 1)

Description

PmrB is a transmembrane kinase. After binding iron (III) ion by binding peptide on extracellular loop, it's intracellular domain gains kinase activity and phosphorylates PmrA (BBa_K1459000).

PmrB C-term is a part of two-component system. When fused with some binding tag, PmrB(N-term), PmrA and pmrC-reporter, it is a viable detecting system.

Notes

We had to use Silver (RFC 23) Standard instead of RFC 10 to allow protein fusions.

Source

We obtained PmrA-PmrB thanks to kindness of prof. He from the University of Chicago.

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1459002/1

Member of these Collections

• iGEM Parts Registry