BBa_K1459009

BBa_K1459009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:32

PmrB(C-term)

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1459009_sequence (Version 1)

Description

PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein.
This part is 'functionally complementary' to PmrA-PmrB(N-term) (BBa_K1459005), in a way that construction of theoretically any detection system is possible by fusing PmrA-PmrB(N-term) with PmrB(C-term) with any binding tag in between two protein domains.

Notes

This part is, much like PmrA-PmrB(N-term), truncated after iron binding tag in PmrB sequence.

Source

We obtained PmrA-PmrB system thanks to kindness of prof. He from the University of Chicago.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1459009/1

Member of these Collections

• iGEM Parts Registry