BBa_K1459011

BBa_K1459011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1459011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grzegorz Ścibisz

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:32

PmrB(N-term)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K1459011_sequence (Version 1)

Description

PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions.
This part was truncated just before the iron binding tag, and PmrB(N-term) is functionally complementar to PmrB(C-term).

Notes

We had to truncate the sequence of PmrB just before start of iron binding tag on the extracellular loop of PmrB.

Source

We obtained PmrA-PmrB sequences thanks to kindness of prof. He of the University of Chicago.

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1459011/1

Member of these Collections

• iGEM Parts Registry