BBa_K1459014

BBa_K1459014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1459014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grzegorz Ścibisz
Date created: 2014-10-12 11:00:00
Date modified: 2015-05-08 01:10:32

Lanthanide Binding Tag



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K1459014_sequence (Version 1)

Description

This is DNA sequence coding a lanthanide binding tag. This one is one of the best described LBTs in literature, with dissociation constants following:
K(La)= 3500 nM
K(Ce)= 950 nM
K(Nd)= 270 nM
K(Eu)= 62 nM
K(Gd)= 84 nM
K(Tb)= 57 nM
K(Dy)= 71 nM
K(Er)= 78 nM
K(Yb)= 100 nM
K(Lu)= 128 nM

Notes

As LBTs sequences are shown in aminoacids, we had to reversely translate this sequence while taking E. coli most often used codons as reference.

Source

This LBT was described in paper

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igem#status
Unavailable
 
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user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1459014/1