BBa_K1461100

BBa_K1461100 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1461100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shunsuke Sumi

Date created: 2014-09-22 11:00:00

Date modified: 2015-05-08 01:10:33

miR-142-3p target binding site

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_K1461100_sequence (Version 1)

Description

After transcription, this cis-repressor sequence makes intramolecular hybridization with RBS and forms stem-loop stem part of which covers RBS. Therefore, when forming stem-loop, following CDS is not translated.
However, if the cognate RNA which called taRNA(trans-activating RNA,BBa_k1461103) exists, this cognate RNA in turn makes hybridization with cis-repressor sequence ,undoes stem-loop and exposes RBS.

Notes

We designed cis-repressor sequence and taRNA sequence to make free energy of most stable formation of stem-loop < free energy of most stable taRNA-annealed RNA by using viennaRNA package.

Source

1.Friedland, A. E., Lu, T. K., Wang, X., Shi, D., Church, G., & Collins, J. J. (2009). Synthetic gene networks that count. science, 324(5931), 1199-1202.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
binding	1,25	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1461100/1

Member of these Collections

• iGEM Parts Registry