Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shunsuke Sumi
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:10:34
RBS-GFP-2miR 142 3p non-bindng sites-2miR 142 5p non-bindng sites-d.term
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1461305_sequence (Version 1) |
Description
This is used for characterization of translation inhibition by miR-142-3p and 5p in HL60 as control. This contains RBS, GFP, 4 miR-142-3p binding sites, 4 miR-142-3p binding sites and double terminator.| Access | Instance | Definition |
| public public public public public public public | BBa_B0034 BBa_E0040 BBa_K1461102 BBa_K1461102 BBa_K1461103 BBa_K1461103 BBa_B0015 | BBa_B0034 GFP BBa_K1461102 BBa_K1461102 BBa_K1461103 BBa_K1461103 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_E0040 BBa_K1461102 BBa_K1461102 BBa_K1461103 BBa_K1461103 BBa_B0015 | 1,12 19,738 747,769 778,800 809,833 842,866 875,1003 | BBa_B0034 GFP BBa_K1461102 BBa_K1461102 BBa_K1461103 BBa_K1461103 BBa_B0015 |