Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ye Peng & Junjie Zhang
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:35
pGAL1+PRK+GBD-ligand+tADH1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1462320_sequence (Version 1) |
Description
This recombinant consists of four parts, the inducible promoter GAL1, the PRK, the ligand of the scaffold GBD and, the teminator ADH1.Notes
PRK is the enzyme that catalytes the reaction from ribulose-5P to ribulose-1,5P, showing high catalytic activity,i.e., a low concentrtion of it is enough for catalyting the reaction. That`s why we utilize a inducible promoter to construct the recombinant.Source
We got pGAL1 and tADH1 from kits, PRK from a synthesis company and, GBD ligand by overlap PCR.| Access | Instance | Definition |
| public public public public | BBa_J63006 BBa_K1462310 BBa_K1462460 BBa_K801012 | GAL1 PRK BBa_K1462460 BBa_K801012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J63006 BBa_K1462310 BBa_K1462460 BBa_K801012 | 1,549 556,1764 1773,1868 1877,2225 | GAL1 PRK BBa_K1462460 BBa_K801012 |