Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM14_Glasgow
Date created: 2014-10-14 11:00:00
Date modified: 2015-05-08 01:10:36
Recombinase Switch With GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1463001_sequence (Version 1) |
Description
PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter so that PhiC31 integrase switches on GFP expression.Notes
The promoter had to face leftwards, the attP and attB sites had to be far enough apart to recombine efficiently and be in head-to-head (inverted repeat) orientation.Source
Composite biobrick consisting of attP, reversed BBa_J23100 promoter, forward facing BBa_B0010 terminator, PhiC31 attB site and the BBa_E0040 GFP gene with a strong BBa_B0034 ribosome binding site.| Access | Instance | Definition |
| public public public public public public public | BBa_K1463040 BBa_K1463030 BBa_K1463501 BBa_B0010 BBa_K1463041 BBa_B0034 BBa_E0040 | attP spacer rev promot BBa_B0010 attB BBa_B0034 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1463040 BBa_K1463030 BBa_K1463501 BBa_B0010 BBa_K1463041 BBa_B0034 BBa_E0040 | 1,54 63,73 82,116 125,204 213,267 276,287 294,1013 | attP spacer rev promot BBa_B0010 attB BBa_B0034 GFP |