BBa_K1467100

BBa_K1467100 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1467100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cara Deal, Jessica Gray

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:38

RFP coding device - Golden Gate Module Flipper

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1467100_sequence (Version 1)

Description

The colonies are clearly red in color under natural light after about 18 hours.
Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.
LacI sensitive
CAP sensitive
This part is a variation of Part:BBa_J04450 with additional flanking sequences recognised by the enzyme BsaI that allow standard GoldenGate Modular Cloning (MoClo) Promoter+5' untranslated leader (Pro+5U) modules to be 'flipped' in a one-pot, one-step, digestion-ligation reaction with BsaI, thus converting the MoClo part to a BioBrick part. When cloning is successful the colonies become white as the RFP sequence is replaced by the new part.

Notes

The sequences flanking the RFP part contain the recognition sequence for BsaI and the (separate) cut-site for BsaI. The cut sites produce 4base pair 5' overhangs compatible with GoldenGate Modular Cloning (MoClo) Promoter+5' untranslated leader (Pro+5U) modules.

Source

Part:BBa_J04450

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1467100/1

Member of these Collections

• iGEM Parts Registry