Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pascal Sartor
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:38
puromycin N-acetyl-transferase
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1470001_sequence (Version 1) |
Description
Puromycin is an antibodic derived from Streptomyces alboniger. The substance affects the cell both in protein translation and mitochondrial protein import. Due to this, it's toxic to many organisms such as bacteria, Trypanosoma and humans.A small part of its structure resembles to aminoacetylated tRNA and is recognized by ribosomes. the nascent chain containing puromycin leads to a translational stop.
Streptomyces alboniger protects itself from puromycin with a acetyl transferase. It uses acetyl-CoA to bind the the acetyl group with the amino group. In this state puromycin cannot be recognized by the ribosom anymore.
This enzyme is a great tool for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis nearly over 99 % of unimfected cells will be dead within tows days.