BBa_K1472001

BBa_K1472001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1472001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Date created: 2014-09-15 11:00:00

Date modified: 2015-05-08 01:10:39

Testing

Details

• Types: DnaRegion
• Roles: sequence_feature; Temporary

Description

Escherichia coli thioesterase without a leader peptide sequence (???TesA)
We have cloned a modified thioesterase gene, ???tesA, which codes for an E. coli thioesterase protein that lacks the N-terminal 26-amino acid signal peptide sequence, and is named here as ???leaderless TesA??? or ???TesA. The ???TesA protein is expected to be expressed exclusively in the cytosol of E. coli to catalyze the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids (Figure 1). ???TesA is predicted to enhance free fatty acid production in the genetically-modified E. coli strain.

Notes

N/A

Source

The genome sequence of Escherichia coli K-12 substr. W3110 is available in GenBank at http://www.ncbi.nlm.nih.gov/gene/12930860.

Other Properties

• igem#experience: None
• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1472001/1

Member of these Collections

• iGEM Parts Registry