BBa_K1472601

BBa_K1472601 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1472601
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lo Hiu Fung, Lau Tsz Kit, Chu Lok Ting, Wu Chun Ting
Date created: 2014-09-15 11:00:00
Date modified: 2015-05-08 01:10:39

Leaderless thioesterase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1472601_sequence (Version 1)

Description

We have cloned a modified thioesterase gene, ???tesA, which codes for an E. coli thioesterase protein that lacks the N-terminal 26-amino acid signal peptide sequence, and is named here as ???leaderless TesA??? or ???TesA. The ???TesA protein is expected to be expressed exclusively in the cytosol of E. coli to catalyze the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids (Figure 1). ???TesA is predicted to enhance free fatty acid production in the genetically-modified E. coli strain.

Notes

N/A

Source

The genome sequence of Escherichia coli K-12 substr. W3110 is available in GenBank at http://www.ncbi.nlm.nih.gov/gene/12930860.

Sequence Annotation Location Component / Role(s)
'tesA
1,549
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1472601/1