Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kai Kin Chi,So Lok Man,Li Lok Yee,Tse Man Chak
Date created: 2014-09-18 11:00:00
Date modified: 2015-05-08 01:10:39
TetR repressible FadL & FadD generator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1472606_sequence (Version 1) |
Description
It is a TetR repressible promoter coupled with fadL, fadD genes and strong RBS. It is inducible by atc to generate fadD and fadL intermembrane proteins. The long-chain fatty acid (LCFA) transporter encoded by fadL will transport LCFA through the outer membrane. The acyl-CoA synthase encoded by fadD gene will further activated LCFA into long chain acyl-CoA and transport them through the inner membrane to the cell.Overexpressing fadD and fadL will render the E.coli strain a higher LCFA intake and extra source of LCFA for its metabolism or other biosynthetic purpose.
This protein generator was used by CityU_HK 2014 team to increase the LCFA uptake for alpha-Linolenic acid production in E.coli.
Notes
The fadD and fadL genes are obtain from PCR amplification but the sequences are the same as the BBa_K861500 and BBa_K861010. This construct is relying on the built in terminator of the biobrick plasmid.Source
It is constructed from standard registry RBS (BBa_B0034, BBa_B0030) and promoter (BBa_R0040). It is coupled with fadD and fadL genes amplified from the genome of E.coli K-12 strains.| Access | Instance | Definition |
| public public public public public | BBa_R0040 BBa_B0030 BBa_K861500 BBa_B0034 BBa_K861010 | p(tetR) BBa_B0030 BBa_K861500 BBa_B0034 BBa_K861010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_B0030 BBa_K861500 BBa_B0034 BBa_K861010 | 1,54 63,77 84,1424 1433,1444 1451,3055 | p(tetR) BBa_B0030 BBa_K861500 BBa_B0034 BBa_K861010 |