BBa_K1472608

BBa_K1472608 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1472608

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lau Tsz Kit

Date created: 2014-09-24 11:00:00

Date modified: 2015-05-08 01:10:39

Oleic acid (18:0) -> alpha-Linolenic acid (18:3-9,12,15)

Details

• Types: DnaRegion
• Roles: Generator; engineered_region

Description

It is a device that contained a promoter (BBa_R0010), RBS (BBa_B0034) for each desaturase gene ∆9,12 and ∆15). When free fatty acids, for example, stearic acid (18:0) enters cell, it is converted into oleic acid (18:1∆9) by ∆9 desaturase, then to linolenic acid (18:2∆9,12) by ∆12 desaturase and finally to alpha-Linolenic acid, ALA (18:3∆9,12,15) by ∆15 desaturase. This device is designated to express under the presence of IPTG.

Notes

The length of the whole device is considered relatively large, it increases the difficulty during cloning procedures.

Source

The three desaturase genes are synthesized according to Thrausochytrid with E. coli optimization.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	engineered_region, feature/BioBrick
end of LacI coding region (inactive)	1,88	CDS, feature/cds
CAP binding site	89,126	non_covalent_binding_site, feature/binding
-35	137,142	feature/promoter, promoter
-10	161,166	feature/promoter, promoter
LacI binding site	166,200	feature/binding, non_covalent_binding_site
start	173,173	start_codon, feature/start
conserved	213,216	conserved_region, feature/conserved
BBa_B0034	209,220	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1472608/1

Member of these Collections

• iGEM Parts Registry