Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan, Zhe
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:10:39
pRha induced CRISPR
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1473009_sequence (Version 1) |
Description
Here we combined a CRISPR system(BBa_K1218011) with an inducible promoter(BBa_K914003).The CRISPR system contains a tracrRNA , a Cas9 protein and a minimal CRISPR array, it functions to knockout target gene. We give this system an inducible promoter so we can decided when to switch if on or off.
Notes
Mutagenesis to remove internal EcoRI site by 2013 Stanford-Brown team.Source
CRISPR is designed by 2013 Stanford-Brown team. They get CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al.| Sequence Annotation | Location | Component / Role(s) |
| tracrRNA part of BBa_K1218011 BBa_K914003 dCas9 + CRISPR array of BBa_K1218011 | 1,325 341,462 472,5011 | feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick |