BBa_K1476003

BBa_K1476003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1476003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Matthew Smith

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:40

PsaD deletion for Synechocystis sp. PCC 6803

Details

• Types: DnaRegion
• Roles: sequence_feature; Other
• Sequences: BBa_K1476003_sequence (Version 1)

Description

This part deletes the PsaD subunit of photosystem I (PSI) in Synechocystis sp. PCC6803.

Notes

The part is designed to contained regions homologous to the psaD gene. As such we had to ensure that the homologous region was of sufficient length to allow for recombination. The part contains two regions homologous to psaD: the first region is homologous to the first 250 bases of psaD, the second region to the last 250 bases of the psaD gene. The centre of the part contains a kanamycin resistance gene, which becomes inserted in the middle of the chromosomal psaD gene during recombination.

Source

Organism: Cyanobacteria
Genus and Strain: Synechocystis sp. PCC 6803
Source: http://genome.microbedb.jp/cyanobase/Synechocystis/genes/slr0737
Important genotype features: none

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
psaD left	1,250	feature/misc, sequence_feature
psaD right	1177,1376	sequence_feature, feature/misc
KanR	281,1096	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1476003/1

Member of these Collections

• iGEM Parts Registry