| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1478002_sequence (Version 1)
|
Description
Multiple domain protein. Domains bind zinc, arsenic, copper and cadmium. Sequences derived from metallothioneins and transcription factors of different organisms. Arsenic, zinc and cadmium binding regions are full length metallothioneins. Zinc binding region derived from a transcritpion factor binding domain. Different protein binding regions are separated with glycerin-serine spacers to improve domain folding. Metal binding regions consists of disulfide bonds. For expression in E. coli disulfide bond forming strains are useful (e. g. Origami).
Notes
Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
Source
Arsenic (CAA06729), zinc (CAD88267) and cadmium (AAN06822) metallothioneins are full length. Copper AAA34542)domain is partial.
