Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGem EPFL 2014
Date created: 2014-08-26 11:00:00
Date modified: 2015-05-08 01:10:42
CxpR & Split IFP1.4 [Cterm + Nterm]
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1486011_sequence (Version 1) |
Description
This part has two fused proteins downstream of an arabinose-inducible promoter: CpxR-IFP[1] and CpxR-IFP[2].This construct was made in all orientations:
- CpxR-IFP[1] + CpxR-IFP[2] (BBa_K1286008)
- IFP[1]-CpxR + CpxR-IFP[2] (BBa_K1486010)
- CpxR-IFP[1] + IFP[2]-CpxR (BBa_K1486011)
Notes
All orientations were made, so all dimerizations could be tested (see main page for biobrick numbers). Flexible linkers were used for each of the fused proteins, and an RBS was put before each one of them.Source
From Michnick Article. To Edit| Access | Instance | Definition |
| public public public public public public public public public | BBa_I0500 BBa_B0034 BBa_K1486000 BBa_K1486004 BBa_K1486006 BBa_B0034 BBa_K1486007 BBa_K1486004 BBa_K1486000 | pBad/araC BBa_B0034 BBa_K1486000 BBa_K1486004 BBa_K1486006 BBa_B0034 BBa_K1486007 BBa_K1486004 BBa_K1486000 |