BtGal1

BBa_K1489001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1489001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iowis Zhu
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:44

Bovine Galectin-1



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1489001_sequence (Version 1)

Description

Soluble beta-galactoside binding protein (galectin) originally from Bos taurus. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of beta-galactosides and initialize signal cascades. Believed to be a dimer in its active form, as is true with most mammalian galectins.

UMaryland 2014 is interested in utilizing this part to investigate whether E. coli can recognize and bind to surface carbohydrates on a marine pathogen. Other uses of this part lie in the recognition of various carbohydrate ligands and potential activation of signal transduction pathways.

Notes

EcoRI site in middle of Bovine Galectin gene was removed, codons were optimized for expression in E. coli.

Source

Sequence generated from Bos taurus genome, reoptimized for E. coli expression.

Sequence Annotation Location Component / Role(s)
ATG Start
TGA Stop
1,3
415,417
feature/start start_codon
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1489001/1