BBa_K1491034

BBa_K1491034 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1491034

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danielle Peters

Date created: 2014-09-25 11:00:00

Date modified: 2015-05-08 01:10:44

Constitutive T7 RNAP with YFPH

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K1491034_sequence (Version 1)

Description

T7 RNA polymerase with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP.

Notes

Constitutive T7 RNAP with YFP for quantification of T7 RNAP

Source

The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the YFP is BBa_K864100

Components

Access	Instance	Definition
public	BBa_J23115	BBa_J23115
public	BBa_B0034	BBa_B0034
public	BBa_K1491030	BBa_K1491030
public	BBa_K1491005	BBa_K1491005

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23115	1,35	BBa_J23115
BBa_B0034	44,55	BBa_B0034
BBa_K1491030	62,2728	BBa_K1491030
BBa_K1491005	2737,3495	BBa_K1491005

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1491034/1

Member of these Collections

• iGEM Parts Registry