Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danielle Peters
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:10:44
Constitutive ER Intein T7 RNAP with YFPH
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1491035_sequence (Version 1) |
Description
T7 RNA polymerase that contains the estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP.Notes
Constitutive ER Intein T7 RNAP with YFP for quantification of T7 RNAPSource
The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the ER Intein is BBa_K1491024, the YFP is BBa_K864100| Access | Instance | Definition |
| public public public public | BBa_J23115 BBa_B0034 BBa_K1491025 BBa_K1491005 | BBa_J23115 BBa_B0034 BBa_K1491025 BBa_K1491005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23115 BBa_B0034 BBa_K1491025 BBa_K1491005 | 1,35 44,55 62,4319 4328,5086 | BBa_J23115 BBa_B0034 BBa_K1491025 BBa_K1491005 |