Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:45
Chalcone Isomerase (CHI) from Petunia
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1497000_sequence (Version 1) |
Description
The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin.
We used the CHI and verified the function in our Naringenin Operon (K1497007).