BBa_K1497000

BBa_K1497000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1497000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:45

Chalcone Isomerase (CHI) from Petunia



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1497000_sequence (Version 1)

    Description

    The Chalcone Isomerse catalyzes the reaction from Naringenin chalcone to Naringenin. This enzyme is non-essential for flavanone pathways, because the reaction takes place independently from CHI in the cytosol. The enzyme independent reaction is catalyzed by an acidic compound. Without CHI the reaction is stereo unspecific. S-Naringenin is the active compound for the most enzymes in the biochemical pathways. To prevent gaining a racemic product and losing 50% yield, CHI is used, because only the enzyme is able to form 100 % S-Naringenin.
    Reaction of the CHI. A proton shift of naringenin chalcone builds naringenin.
    We used the CHI and verified the function in our Naringenin Operon (K1497007).

    Notes

    Original Sequence from Petunia

    Source

    Petunia Hybrida

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1497000/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)