BBa_K1497021

BBa_K1497021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1497021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:10:45

Naringenin sensor (FdeR) with mKate as reporter

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1497021_sequence (Version 1)

Description

We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae.
You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors:
GFP fluorescence K1497020
mKate (DsRed variant) fluorescence K1497021
CFP fluorescence K1497022

Notes

d

Source

d

Components

Access	Instance	Definition
public	BBa_K1497019	BBa_K1497019
public	BBa_K1055000	BBa_K1055000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1497019	1,1214	BBa_K1497019
BBa_K1055000	1221,1937	BBa_K1055000

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1497021/1

Member of these Collections

• //cds/reporter
• //cds/reporter/rfp
• //function/sensor
• iGEM Parts Registry