BBa_K1498000

BBa_K1498000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1498000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Charlotte Green
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:46

kerUS keratinase from Brevibacillus brevis



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1498000_sequence (Version 1)

Description

Coding region of kerUS keratinase from Brevibacillus brevis with signal for extracellular secretion, which is cleaved following secretion

Codes for a 45.9kDa (predicted)pre-processed protein, cleaved to 29kDa active protein

Notes

codon optimisation for E. coli, ensuring no BioBrick restriction enzyme sites were present

Source

is the codon optimised (for E. coli) kerUS gene from:
Jaouadi, N. Z., Rekik, H., Badis, A., Trabelsi, S., Belhoul, M., Yahiaoui, A. B., ??? Jaouadi, B. (2013). Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PloS one, 8(10), e76722. doi:10.1371/journal.pone.0076722

Genbank Number: KC152966

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1498000/1