Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1498000_sequence (Version 1)
|
Description
Coding region of kerUS keratinase from Brevibacillus brevis with signal for extracellular secretion, which is cleaved following secretion
Codes for a 45.9kDa (predicted)pre-processed protein, cleaved to 29kDa active protein
Notes
codon optimisation for E. coli, ensuring no BioBrick restriction enzyme sites were present
Source
is the codon optimised (for E. coli) kerUS gene from:
Jaouadi, N. Z., Rekik, H., Badis, A., Trabelsi, S., Belhoul, M., Yahiaoui, A. B., ??? Jaouadi, B. (2013). Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PloS one, 8(10), e76722. doi:10.1371/journal.pone.0076722
Genbank Number: KC152966