Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jovita Byemerwa
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:46
Endo-1,4-beta-glucanase (cellulase gene)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1499501_sequence (Version 1) |
Description
Endo-1,4-&-beta-glucanase (EG 1) is an enzyme that breaks down the 1,4-beta-D-glycosidic linkages in cellulose to produce glucose. The coding sequence of this gene was from Neisseria Sicca bacterium that uses cellulose acetate as its sole carbon source. This enzyme is specific for 1,4-beta linkages in cellulose.Notes
Since the gene is usually secreted by the original bacterium (Neisseria Sicca), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene.Source
Genomic sequence of Neisseria Sicca SB| Sequence Annotation | Location | Component / Role(s) |
| Cellulase Metal-affinity tag | 1,1374 1378,1398 | CDS feature/cds tag feature/tag |