BBa_K1499502

BBa_K1499502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1499502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jovita Byemerwa
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:10:46

Cellulose Acetate Esterase (Cae)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1499502_sequence (Version 1)

Description

Cellulose Acetate Esterase is an enzyme that de-acetylates cellulose acetate by hydrolyzing the acetyl ester moieties in cellulose acetate. The gene sequence is obtained from a bacterium, Neisseria Sicca that uses cellulose acetate as its sole source of carbon. Purified Cellulose Acetate Esterase has a high activity on Cellulose Acetate, but it has been shown to have activity on p-nitrophenyl acetate and some other p-nitrophenyl esters. (Original paper https://www.jstage.jst.go.jp/article/bbb/63/10/63_10_1708/_pdf)
Since industrially made cellulose acetate is a plastic that tends to take a long time to biodegrade in the environment, the use of this enzyme could help in rapid degradation of cellulose acetate by removing the acetate groups. This part could be used specifically for de-acetylating cellulose acetate and other related esters.

Notes

Neisseria Sicca secretes the enzyme extracellularly and hence it has a signal sequence to aid for secretion. In order to use the gene in E.coli, we removed the signal sequence of Neisseria Sicca and added an E.coli specific secretion tag. In addition to that, we added a his-tag at the c'terminus end of the protein to aid purification of the protein.

Source

Genomic sequence of Neisseria Sicca

Sequence Annotation Location Component / Role(s)
Signal sequence
Esterase
His Tag
94,184
189,1446
1447,1468
feature/dna sequence_feature
sequence_feature feature/dna
feature/tag tag
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1499502/1