BBa_K1500007

BBa_K1500007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1500007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ryan Beckner

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:46

IPTG->GFP+mutH(E56A)

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1500007_sequence (Version 1)

Description

This part contains GFP and the mutH(E56A) protein under the control of an IPTG-inducible promoter. MutH(E56A) has been shown to have mutagenic activity E. coli when expressed.

Notes

The IPTG+GFP portion of the plasmid was first constructed followed by the mutH(E56A)

Source

MutH was isolated from MG1655 genomic DNA and underwent quikchange mutagenesis to become mutH(E56A)

Components

Access	Instance	Definition
public	BBa_K1500002	BBa_K1500002
public	BBa_B0032	BBa_B0032
public	BBa_K1500996	BBa_K1500996

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1500002	1,946	BBa_K1500002
BBa_B0032	955,967	BBa_B0032
BBa_K1500996	974,1663	BBa_K1500996

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1500007/1

Member of these Collections

• iGEM Parts Registry