Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rivka Baker Keusch
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46
ParoF--mCherry--EmrR
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1500012_sequence (Version 1) |
Description
A plasmid that contains the tyrosine-repressible promoter ParoF, followed by an RBS (RBS.3, medium strength), mCherry-LVA as a reporter gene directly downstream, followed by an RBS and the mutator EmrR. The plasmid is an actuator module of our project which contains an optimized promoter, a reporter, and a mutators. The transcription factor TyrR can bind to ParoF and repress it when itself bound to tyrosine.Notes
NASource
various genes from MG1655| Access | Instance | Definition |
| public public | BBa_K1500001 BBa_K1500995 | BBa_K1500001 BBa_K1500995 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1500001 BBa_K1500995 | 1,950 957,1487 | BBa_K1500001 BBa_K1500995 |