Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin Yang
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46
ParoF-mCherry backbone
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1500001_sequence (Version 1) |
Description
A plasmid that contains the tyrosine-repressible promoter ParoF, followed by an RBS (RBS.3, medium strength) and mCherry-LVA as a reporter gene directly downstream. We used it to insert our combinations of mutator genes directly downstream of the ParoF promoter, forming the actuator module of our project which contains an optimized promoter, a reporter, and one or more mutators. The transcription factor TyrR can bind to ParoF and repress it when itself bound to tyrosine.Notes
In order to insert mutator genes downstream of the mCherry-LVA, we would cut open the two downstream restriction sites, with corresponding cuts on the mutator genes, before ligating the insert and this plasmid together.Source
mCherry-LVA (BBa_J06505) was used and came from the registry plasmid. ParoF was cloned from wild-type DH5-alpha E. coli genomic sequence. RBS.3 (BBa_B0032) sequence originally came from the registry and was designed directly into primer overhangs for mCherry-LVA.| Access | Instance | Definition |
| public public public | BBa_K1500999 BBa_B0032 BBa_J06505 | BBa_K1500999 BBa_B0032 mCherry |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1500999 BBa_B0032 BBa_J06505 | 1,200 209,221 228,950 | BBa_K1500999 BBa_B0032 mCherry |