BBa_K1500013

BBa_K1500013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1500013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin Yang

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:46

ParoF--mCherry--dam

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1500013_sequence (Version 1)

Description

Dam is involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions. It requires no mutations and simple overexpression is sufficient to cause its mutagenic activity. In this plasmid, it is placed downstream of the tyrosine-repressible promoter ParoF and reporter gene mCherry to fulfill our goals to improving an established system of directed evolution.

Notes

NA

Source

Dam was cut from K1500994 and inserted into ParoF--mCherry backbone registry plasmid K1500001.

Components

Access	Instance	Definition
public	BBa_K1500001	BBa_K1500001
public	BBa_B0032	BBa_B0032
public	BBa_K1500994	BBa_K1500994

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1500001	1,950	BBa_K1500001
BBa_B0032	959,971	BBa_B0032
BBa_K1500994	978,1814	BBa_K1500994

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1500013/1

Member of these Collections

• iGEM Parts Registry