Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ryan Beckner
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:46
paroF->MCherry-MutH(E56A)
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1500014_sequence (Version 1) |
Description
This biobrick contains mutH() and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH() and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached. mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.Notes
NASource
paroF, mutH are genomic XL1Blue, mCherry is from distribution kit.| Access | Instance | Definition |
| public public public | BBa_K1500001 BBa_B0032 BBa_K1500996 | BBa_K1500001 BBa_B0032 BBa_K1500996 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1500001 BBa_B0032 BBa_K1500996 | 1,950 959,971 978,1667 | BBa_K1500001 BBa_B0032 BBa_K1500996 |