Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jane Shmushkis, Amey Vrudhula
Date created: 2014-10-09 11:00:00
Date modified: 2016-01-13 10:36:51
RBS+mntH+mCerulean
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1505002_sequence (Version 1) |
Description
The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. mntH was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The MntH protein and mCerulean protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of MntH and for better visualization of successful transformation. The original use is to express MntH in Magnetospirillum magneticum strain AMB-1.Notes
We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCerulean protein is expected to improve the folding of MntH in AMB-1.Source
RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. MntH is from part BBa_K1505000. mCerulean is from part BBa_K133066.| Sequence Annotation | Location | Component / Role(s) |
| rbs (from pmsp3) mntH ggggs linker mCerulean | 19,46 67,1302 1303,1317 1318,2034 | ribosome_entry_site feature/rbs CDS feature/protein sequence_feature feature/misc CDS feature/protein |