Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Clay Swackhamer, Sam Krug, Ashlee Smith, Emily Sileo
Date created: 2014-08-04 11:00:00
Date modified: 2015-05-08 01:10:47
Superfolder GFP with only rare codons
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1506000_sequence (Version 1) |
Description
This part is a reporter gene for green florescent protein. It has the same amino acid sequence as superfolder GFP (part_) but is comprised only of rare codons.Notes
The codon optimization that was used when designing this sequence was based on the codon usage profile for E. coli across its entire genome. To produce any particular amino acid, E. coli can usually use one of several codons, because of codon redundancy. Previous researchers compiled a table showing the frequency with which each codon appears in the entire genome and organized the data to show which codons are the most commonly and most rarely used to produce any given amino acid. To create this GFP, the rarest possible codon was used for each amino acid in the protein.Source
This is a purely synthetic part.| Sequence Annotation | Location | Component / Role(s) |
| start codon rare GFP cds two stop codons Leader Sequence same for all 5 variant GFPs) | 1,3 64,774 774,780 4,63 | feature/start start_codon CDS feature/cds stop_codon feature/stop feature/misc sequence_feature |