Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Clay Swackhamer, Sam Krug, Ashlee Smith, Emily Sileo
Date created: 2014-08-04 11:00:00
Date modified: 2015-05-08 01:10:47
Superfolder GFP with only common codons
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1506001_sequence (Version 1) |
Description
This part is a reporter gene producing green florescent protein. It is translated to the same amino acid sequence as superfolder GFPNotes
This GFP codes for the same green florescent protein as superfolder GFP, but it is comprised only of common codons. Previous researchers have determined through a statistical analysis of the entire genome of E. coli that some degenerate codons occur more often in protein coding sequences and some are more infrequent. These are referred to as common and rare codons. The hypothesis that common codons will lead to higher expression of proteins is based on the idea that cellular DNA has become optimized through evolution to efficiently translate proteins necessary to their survival. For each amino acid coded by this GFP, the most commonly occurring codon in the genome of E. coli that specifies that amino acid was used, thus creating a coding sequence of only common codons.Source
This part is entirely synthetic.| Sequence Annotation | Location | Component / Role(s) |
| start codon two stop codons common GFP CDS Leader Sequence same for all 5 variant GFPs) | 1,3 775,780 64,774 4,63 | feature/start start_codon stop_codon feature/stop feature/cds CDS feature/misc sequence_feature |