Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fahmi Dwilaksono, Indah Nurulita, Rian Adha Ardinata, Mochammad Isro Alfajri, Arief Budi Witarto
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:47
IIA(Glc) - E.coli glucose-specific phosphotransferase enzyme IIA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1508002_sequence (Version 1) |
Description
Phosphotransferase system (PTS) is a major carbohydrate active -transport system, which is executed by a cascade of protein rally. For glucose uptake, IIA(Glc) is the only enzyme specific to glucose transport. We measure the concentration of glucose in the medium using catabolite repression mechanism toward lacI promoter activity upstream of a reporter gene, mRFP. We expect by over-expressing IIA(Glc) in E. coli, we will obtain different range of glucose standard curve in our measurement system.Notes
The gene coding for IIA(Glc) from E.coli - crr - was isolated by colony PCR using forward primer which contains with restriction sites of EcoRI and XbaI and reverse primers which contains restriction site for SpeI and a double stop codon.Source
Escherichia coli DH5alpha genome| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon IIA(Glc) coding region TAA TAA stop codon | 1,3 1,513 508,513 | start_codon feature/start CDS feature/cds stop_codon feature/stop |