| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K1509001_sequence (Version 1)
|
Description
smt O-P is a promoter which has two inverted repeats, designated S1/S2 and S3/S4, in the overlapping promoter/operator sites between gene smtA and gene smtB. The full promoter/operator DNA binds two SmtB dimmers forming a S1/S2-SmtB:SmtB-S3/S4 bridge complex. After binding with smtB, smt O-P represses the expression of downstream genes.
Notes
We got smt O-P and smtA, a fragment coding MT-like to sequester metal ions from smt locus by PCR and overlaped two fragments,smtB-smt O-P and pigment gene.Binding with smtB, smt O-P represses the expression of reporter genes.In the presence of heavy metal,the pigment and SmtA can expression normally.
Source
smt O-P is a part of smt locus from Synechococcus PCC7942.
