BBa_K1509003

BBa_K1509003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1509003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tong Li
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:10:47

CP25 is a highly active,constitutive promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1509003_sequence (Version 1)

Description

The synthetic CP25 promoter was designed to be constitutive. CP25 is a stronge promoter in Lactococcus lactis by Jensen and Hammer.In the article,they measure the activities of the CP promoter in E. coli.The promoter activities were assayed from the expression of a reporter gene (lacLM) encoding β-galactosidase transcribed from the different synthetic promoter clones on the promoter cloning vector pAK80.The result suggests CP25 is the strongest promoter.

Notes

CP25 was used as the strong constitutive promoter to regulate the expression of the gene CDS7.

Source

We synthesized CP25 from a library of synthetic promoters for Lactococcus lactis.

Sequence Annotation Location Component / Role(s)
CP25
1,59
feature/promoter promoter
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1509003/1