Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei-Tai Chen
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:47
sRNA targets histidine kinase 11 in S.mutans
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1510006_sequence (Version 1) |
Description
veg promoter(constitutive promoter in S.mutans+ Histidine kinase 11 sRNA+ MicC scaffold+ terminator. Constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.This part will transcibe into sRNA, and sRNA will bind to mRNA of HK11, prevent HK11 mRNA from translating. Because histidine kinase 11 is a biofilm formation-related protein, this part is created to decrease biofilm formation.
Notes
Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)Source
constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.
| Access | Instance | Definition |
| public public public public | BBa_K1510100 BBa_K1510101 BBa_K1510103 BBa_K1510104 | BBa_K1510100 BBa_K1510101 BBa_K1510103 BBa_K1510104 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1510100 BBa_K1510101 BBa_K1510103 BBa_K1510104 | 1,50 59,82 91,169 178,199 | BBa_K1510100 BBa_K1510101 BBa_K1510103 BBa_K1510104 |