Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei-Tai Chen
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:47
sRNA targets G protein mRNA in S.mutans
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1510007_sequence (Version 1) |
Description
veg promoter(constitutive promoter in S.mutans+ G protein sRNA+ MicC scaffold+ terminator. Constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.This part will transcibe into sRNA, and sRNA will bind to mRNA of SGP(S.mutans G protein), prevent SGP mRNA from translating. Because G protein is a biofilm formation-related protein, this part is created to decrease biofilm formation.
Notes
Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)Source
constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.
| Sequence Annotation | Location | Component / Role(s) |
| Veg promoter sRNA targets G protein MicC scaffold terminator | 1,36 42,65 66,144 145,157 | promoter feature/promoter feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding stop_codon feature/stop |