| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1510011_sequence (Version 1)
|
Description
By putting strong constitutive promoter, J23100 in front of yebF and its ribosomal biding site. The recombinant endolysin should be secreted. By adding this part, we are able to have recombinant endolysin form Phage M!02 outside E.coli. In our circuit, we use this circuit to see if endolysing from specific phage can be expressed by E.coli and successfully kill Strepptococcus Mutans.
Notes
The endolysin sequence from phage M102 contains a Spe1 cutting site, therefore, we design primers to make point mutation of the sequence in order to produce the same amino acid without the risk of being cut during circuit construction.
Source
J23100 is a strong constitutive promoter from 2014 igem distribution. The coding region, yebF and endolysin come from different origin. YebF is from E.coli K12, while endolysin is from the 19th opening reading frame of Streptococcus Mutans phage M102. Finally, B0015 is a double terminator from igem distribution.
