Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kao Jung Chang
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:48
Truncated lysostaphin coding circuit
| Types | DnaRegion |
| Roles | Conjugation oriT |
| Sequences | BBa_K1510904_sequence (Version 1) |
Description
we use truncated lysostaphin coding sequence(designed by HIT-Harbin 2012 igem team) to reach our goal deplete biofilm formed by streptococcus mutans. YebF,a signal protein, would be secreted outside E.coli to make protein works more accurately.Notes
To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.Source
The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction.The truncated lysostaphin coding sequence is from 2013 igem distribution. Finally, the terminator B0015 is from 2014 igem distribution| Access | Instance | Definition |
| public public public public | BBa_J23100 BBa_K1510009 BBa_K748002 BBa_B0015 | BBa_J23100 BBa_K1510009 BBa_K748002 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_K1510009 BBa_K748002 BBa_B0015 | 1,35 44,584 591,1331 1340,1468 | BBa_J23100 BBa_K1510009 BBa_K748002 BBa_B0015 |