BBa_K1510929

BBa_K1510929 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1510929

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wang,Ching-Yun

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:48

Circuit injected in Streptococcus mutans that allows S.m. yield N-Acyl homoserine lactone.

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1510929_sequence (Version 1)

Description

This circuit mainly contains luxI gene which stands for AHL synthetase coding sequence. AHL is the communicator signal we adopt to connect Streptococcus mutans and Escherichia coli with S.m. killing gene.
While the chemical signal AHL is successfully generated, the killing gene(Endolysin coding sequence) inside E.coli would be triggered and begins to destruct the cell wall of our target harmful germ, Streptococcus mutans.

Notes

We have to design PCR primers of the constitutive promoter region of Streptococcus mutans to get the constitutive expression of the LuxI gene.

Source

Constitutive Promoter: Comes from Streptococcus mutans genome.
LuxI gene: BBa_C0061
Terminator:BBa_B0015

Components

Access	Instance	Definition
public	BBa_C0061	luxI
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_C0061	1,618	luxI
BBa_B0015	652,780	BBa_B0015

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1510929/1

Member of these Collections

• iGEM Parts Registry