BBa_K1521001

BBa_K1521001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1521001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danilo Keiji Zampronio

Date created: 2014-09-30 11:00:00

Date modified: 2015-05-08 01:10:49

RBS SpoVG (K143021) + lasR

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1521001_sequence (Version 1)

Description

This is an assembly os the biobricks SpoVG (K143021) for Bacillus subtilis and the LasR protein cds. Note that the lasR sequence was obtained through PCR of the part C0079 and that its LVA tag was also removed.

Although the RBS was designed for B. subtilis also works on E. coli.

This protein requires an HSL (homoserine lactone) produced by the gene lasI (C0078) to work appropriately. If you want the lasI is already available with RBS, cds, terminator and a promoter responsive to LasR (K081001).

Notes

This protein requires an HSL (homoserine lactone) produced by the gene lasI (C0078) to work appropriately. If you want the lasI is already available with RBS, cds, terminator and a promoter responsive to LasR (K081001).

Source

This part was obtaind through PCR of the part C0079. We also removed the LVA tag and added the RBS SpoVG (K143021).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	1,12	ribosome_entry_site, feature/rbs
cds	19,738	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1521001/1

Member of these Collections

• iGEM Parts Registry