BBa_K1526001

BBa_K1526001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1526001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ruth Haley

Date created: 2014-10-04 11:00:00

Date modified: 2015-05-08 01:10:50

Gsh1*: Mutated Glutamate-Cysteine Ligase(Gsh1) from Saccharomyces cerevisiae

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K1526001_sequence (Version 1)

Description

Glutamate-Cysteine Ligase(previously known as Gamma glutamylcysteine synthetase) catalyzes the first step in glutathione (GSH) biosynthesis;

L-glutamate + L-cysteine + ATP -> gamma-glutamyl cysteine + ADP + Pi

Expression is induced by oxidants, cadmium, and mercury. Protein abundance increases in response to DNA replication stress. (http://biocyc.org/YEAST/NEW-IMAGE?type=GENE-IN-MAP-IN-PWY&object=YJL101C)

Gsh1* can be used to overproduce cysteine to make gamma-glutamyl cysteine, which is the monomer that forms phytochelatins (n=10-20). In order to do this, glutamate-cysteine ligase is needed to catalyse the reaction. We can overexpress GSH1* using the pYodA(BBa K896008) promoter.

Notes

The original gene(Gsh1/GshA) was mutated to obtain Gsh1*.

Source

http://nar.oxfordjournals.org/content/14/11/4393.full.pdf

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	7,18	ribosome_entry_site, feature/rbs
Start Codon	25,27	feature/start, start_codon
Gsh1*	25,1581	CDS, feature/cds
Stop	1579,1581	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1526001/1

Member of these Collections

• iGEM Parts Registry