Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrik D
Date created: 2014-10-12 11:00:00
Date modified: 2015-05-08 01:10:51
Bovine beta casein (B variant) with full alpha-factor yeast secretion signal
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1531004_sequence (Version 1) |
Description
Beta casein is one of four major caseins (cheese proteins) found in bovine cheese. Together with Kappa casein, it is necessary and sufficient to create casein micelles.At least 12 different genetic variants of bovine Beta casein have been identified and characterized. The B allele used here is associated with improved milk coagulation.
The coding sequence for this gene is preceded by a secretion signal derived from the yeast mating pheromone alpha-factor in Saccharomyces cerevisiae, facilitates secretion of heterologous proteins in yeast. We used a full length form of the full alpha-factor protein with kex and ste13 protease cleavage sites (FAKS). (www.pnas.org/content/81/15/4642.short). In addition, the entire construct is flanked by SapI restriction sites, for cloning into DNA2.0's Electra system.
Notes
The DNA sequence was codon optimized for expression in S. cerevisiae, using IDT's codon optimization tools, followed by manual editing of a few codons to avoid specific restriction sites.Source
Bos taurus sequence, from http://www.ncbi.nlm.nih.gov/protein/AAI11173.1| Sequence Annotation | Location | Component / Role(s) |
| Sap1 start codon alpha factor yeast secretion signal bovine Beta casein stop codon SapI | 1,11 9,11 12,275 276,905 903,905 906,916 | feature/misc sequence_feature feature/start start_codon tag feature/tag feature/cds CDS stop_codon feature/stop sequence_feature feature/misc |