BBa_K1539001

BBa_K1539001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1539001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Coleen Tran

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:52

HPromoter->HRBS->mCherry

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1539001_sequence (Version 1)

Description

Coding sequence has a high efficiency promoter followed by a high efficiency RBS to express fluorescent protein mCherry.

Notes

RBS primer construction had to maintain EcoRI and XbaI site. Promoter primer construction had to eliminate the EcoRI site but once ligated to the backbone, this site was reestablished.

Source

mCherry from BBa_J0650
Promoter and RBS originated from primers

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1539055	1,35	promoter, feature/promoter
BBa_K1539021	44,56	ribosome_entry_site, feature/rbs
BBa_J06504	63,773	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1539001/1

Member of these Collections

• iGEM Parts Registry