BBa_K1539002

BBa_K1539002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1539002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Coleen Tran
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:52

LPromoter->LRBS->mCherry



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1539002_sequence (Version 1)

Description

This coding sequence contains a low efficiency promoter followed by a low efficiency RBS allowing for the expression of mCherry

Notes

RBS primer construction needed to maintain the EcoRI and XbaI site. The promoter primer design could not maintain the EcoRI site but does contain the XbaI sequence. EcoRI was reestablished when the entire coding sequence was ligated to the backbone.

Source

mCherry from BBa_J06504
RBS and promoter from created primers

Sequence Annotation Location Component / Role(s)
BBa_K1539089
BBa_K1539034
BBa_J06504
1,35
44,56
63,773
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/cds CDS
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1539002/1